Cloning and
Expression of Gynecophoral Canal Protein Gene of Schistosoma japonicum
(Chinese strain)
JIN Ya-Mei1,2, LIN Jiao-Jiao1,
FENG Xin-Gang, ZHANG Liang1, WU Xiang-Fu2,ZHOU Yuan-Cong2,CAI
You-Min£±*
( 1Shanghai Institute of Animal Parasitology, Chinese Academy of
Agricultural Science, Key Laboratory of Animal Parasitology, Ministry of
Agriculture of China, Shanghai 200232,China; 2Institute
of Biochemistry and Cell Biology, Shanghai Institute for Biological
Sciences,the Chinese Academy of Sciences, Shanghai 200031,China )
Abstract A 1949 bp
cDNA fragment was amplified by RT-PCR from adult Schistosoma japonicum
(Chinese strain) mRNA with 3 pair of primers that were designed according to
published SmGCP gene encoding gynecophoral canal protein of Schistosoma
mansoni and SjGCP1 gene encoding the conservative region of
gynecophoral canal protein of Schistosoma japonicum.Sequence analysis
indicated that this fragment, named SjGCP,with 85% identity to SmGCP,
contained a complete open reading frame (ORF) of gynecophoral canal protein
gene of Schistosoma japonicum (Chinese strain).The amino acid sequence
shared 83.7% identity with gynecophoral canal protein of Schistosoma mansoni.This
fragment was cloned into the expression vector pET28c(+) and subsequently
expressed in Escherichiacoli.SDS-PAGE revealed that the molecular weight
of this expressed product was 80 kD.Western blotting showed that the
recombinant protein reacted well with the rabbit serum immunized with Sj
worm antigen,indicating that this expressed product had good antigenicity.
Key words Schistosoma japonicum; gynecophoral
canal protein; gene cloning; gene expression
*Corresponding author:
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